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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD117 C-Kit Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD117 (c-Kit) Antibody
1129085 25 µg $90.00       
1129090 100 µg $190.00       
Clone: 2B8
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse bone marrow mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of acetone fixed frozen sections2. The 2B8 antibody does not block c-Kit activity.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
4. Charles N, et al. 2010. Nat. Med. 16:701.  PubMed (FC)
5. Shade KT, et al. 2015. J Exp Med. 212:457. PubMed

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 647



Description:

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand Receptor: Stem Cell Factor (SCF)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.


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