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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse Ly-6A/E Sca-1 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse Ly-6A/E (Sca-1) Antibody
1140575 25 µg $90.00       
1140580 100 µg $190.00       
Clone: D7
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: IL-2-dependent mouse T-cell line (CTL-L)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
3. Codias EK, et al. 1990. J. Immunol. 144:2197.
4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
5. Codias EK, et al. 1990. J. Immunol. 145:1407.
6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
8. Fleming T, et al. 1994. J. Immunol. 153:1955.
9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
10. Umland O, et al. 2007. J. Immunol. 178:4147.
11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294. 

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with D7 Alexa Fluor® 488



Description:

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Other Names: Sca-1
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Antigen References:

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.


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