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Safety Data Sheet (SDS) FITC Anti-mouse/human CD44 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse/human CD44 Antibody
1115105 25 tests $30.00       
1115025 50 µg $45.00       
1115030 500 µg $90.00       
1115110 100 tests $90.00       
Clone: IM7
Isotype: Rat IgG2b, κ
Reactivity: Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: test size: lot-specific; µg size: 0.5 mg/ml
Storage & Handling: The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
8. Lee JW, et al. 2006. Nature Immunol. 8:181.
9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
12. Wang XY, et al. 2008. Blood 111:2436. PubMed
13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
14. Yamazaki J, et al. 2009. Blood PubMed
15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
20. Sandmaier BM, et al. 1998. Blood 91:3494.
21. Lu X, et al. 2015. J Immunol. 194:2011. PubMed

C57BL/6 splenocytes stained with IM7

C57BL/6 splenocytes stained with IM7 FITC

Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with IM7 FITC



Description:

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.