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Safety Data Sheet (SDS) PE/Cy5 Anti-mouse NK-11 Antibody     Product Data Sheet (PDF)    
PE/Cy5 Anti-mouse NK-1.1 Antibody
1143575 25 µg $90.00       
1143580 100 µg $195.00       
Clone: PK136
Isotype: Mouse IgG2a, κ
Reactivity: Mouse
Immunogen: NK-1+ cells from mouse spleen and bone marrow
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, and immunofluorescence microscopy11.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cy5.



Description:

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Other Names: NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Antigen References:

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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