The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, and immunofluorescence microscopy11.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP) 2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP) 3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt) 4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete) 5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete) 6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. 7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block) 8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143. 9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) 10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete) 11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF) 12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed 13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed 14. Bankoti J, et al.2010. Toxicol. Sci.115:422. (FC) PubMed 15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cy5.
NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
NKR-P1 gene family
NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
NK cell activation, IFN-γ production, cytotoxic granule release
1. Lanier LL. 1997. Immunity 6:371. 2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613. 3. Koo GC, et al. 1986. J. Immunol. 137:3742. 4. Giorda R, et al. 1991. J. Immunol. 147:1701.
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