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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-human/mouse/rat PCNA Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-human/mouse/rat PCNA Antibody
2139545 25 tests $100.00       
Clone: PC10
Isotype: Mouse IgG2a, κ
Reactivity: Human, Mouse, Rat, Cross-Reactivity: Other species
Immunogen: Recombinant rat PCNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
IF

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis.pdf" target="_blank">Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Ogata K, et al. 1985. J. Immunol. 135:2623.
2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
5. Yu C, et al. 1991. Histopathology 19:29.
6. Wilkins B, et al. 1992. J. Pathol. 166:45.
7. Yang W, et al. 1996. Human Pathol. 27:70.
8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
10. Fulvio MD, et al. 2006. Oncogene 25:3932.
11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
12. Horton NC, et al. 2013. PLoS One. 8:e59552. PubMed.

MOLT-4 cells fixed in 70%

MOLT-4 cells fixed in 70% ethanol then stained with PC10 Alexa Fluor® 488 or MOPC-173 Alexa Fluor® 488





Description:

The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.

Other Names: Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Structure: DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution: Nuclear, all proliferating cells
Function: RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Ligand Receptor: Ubiquitination, Sumoylation
Interaction: PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Antigen References:

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.