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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD43 Activation-Associated Glycoform Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD43 Activation-Associated Glycoform Antibody
1206050 100 µg $190.00       
Clone: 1B11
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse WEHI 274.3 myeloid tumor cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
5. Chu VT, et al. 2007. J. Immunol. 179:5947.
6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 1B11 Alexa Fluor® 488



Description:

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.

Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand Receptor: CD54, Siglec-1
Antigen References:

1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.


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