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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD11a Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD11a Antibody
1105565 25 µg $90.00       
1105570 100 µg $190.00       
Clone: M17/4
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C57BL/6 mouse splenic secondary cytotoxic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647 .
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of cell-cell adhesion1,2 and FOXP3 expression5, and immunohistochemical staining of acetone-fixed frozen sections3. The M17/4 antibody does not block the binding of 2D7 antibody to CD11a.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
5. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with M17/4 Alexa Fluor® 647



Description:

CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Other Names: αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Structure: Integrin family, associates with integrin β2 (CD18), 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.