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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD18 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD18 Antibody
1107070 100 µg $190.00       
Clone: M18/2
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse CTL glycoprotein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC , IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Application References:

1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with M18/2 Alexa Fluor® 647



Description:

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Other Names: integrin β2, β2 integrin, ITGB2
Structure: Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution: All leukocytes
Function: Adhesion
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.