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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD24 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD24 Antibody
1109075 25 µg $90.00       
1109080 100 µg $190.00       
Clone: M1/69
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with M1/69 Alexa Fluor® 488



Description:

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Other Names: Heat Stable Antigen (HSA), Ly-52, Nectadrin
Structure: GPI-linked sialoglycoprotein, 35-45 kD
Distribution: B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
Function: Regulates B cell proliferation and differentiation
Ligand Receptor: P-selectin, CD24
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.