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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse H-2D D Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse H-2D d Antibody
1153060 100 µg $190.00       
Clone: 34-2-12
Isotype: Mouse (C3H/HeJ) IgG2a, κ
Reactivity: Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc. Does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).
Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Ozato K, et al. 1982. Transplantation 34:113.
2. McCluskey J, et al. 1985. Cell 40:247.
3. McCluskey J, et al. 1986. J. Immunol. 136:1472.
4. Kane KP. 1994. J. Exp. Med. 179:1011.
5. Daniels BF, et al. 1994. J. Exp. Med. 180:687.
6. Nikolic B, et al. 1999. J. Immunol. 162:3402.
7. Ma XT, et al. 2006. Cancer Research 66:1169.
8. Matsumoto N, et al. 1998. Immunity 8:245.

BALB/c mouse splenocytes stained with

BALB/c mouse splenocytes stained with 34-2-12 Alexa Fluor® 647



Description:

The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Dd haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.


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