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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse I-Ab Abetab Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse I-Ab (Aβb) Antibody
1172035 25 µg $90.00       
Clone: 25-9-17
Isotype: Mouse (C3H) IgG2a, κ
Reactivity: Mouse b haplotype, Cross-reacts with alloantigens of H-2d,p,q haplotypes
Immunogen: C3H.SW splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Wall KA, et al. 1983. J. Immunol. 131:1056. (IP)
2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747.
3. Ozato K, et al. 1981. J. Immunol. 126:317.
4. Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC)
5. Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC)
6. Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 25-9-17 Alexa Fluor® 488



Description:

The 25-9-17 antibody reacts with the beta chain of the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 25-9-17 antibody cross-reacts with alloantigens of the H-2d, p, q haplotypes.

Other Names: MHC class II
Structure: ß chain of I-Ab MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.


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