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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse I-Ad Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse I-Ad Antibody
1175040 100 µg $190.00       
Clone: 39-10-8
Isotype: Mouse (C3H.SW) IgG3, κ
Reactivity: Mouse d and g7 haplotypes, Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s)
Immunogen: (C3H x BALB/c)F1 mouse cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Hiramine C, et al. 1995. Cell. Immunol. 160:157.
2. Wang Z, et al. 2004. J. Immunol. 172:5924.
3. Ma XT, et al. 2006. Cancer Research 66:1169.
4. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
5. Tian C, et al. 2007. J. Immunol. 179:6762.

Balb/c mouse splenocytes stained with

Balb/c mouse splenocytes stained with 39-10-8 Alexa Fluor® 488



Description:

The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.

Other Names: MHC class II
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Wall KA, et al. 1983. J. Immunol. 131:1056.
4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.


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