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Safety Data Sheet (SDS) PE Anti-mouse IgE Antibody     Product Data Sheet (PDF)    
PE Anti-mouse IgE Antibody
2634535 50 µg $95.00       
2634540 200 µg $245.00       
Clone: RME-1
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

This clone also recognizes IgE when bound to FcεRI.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Jeong KT, et al. 2012. Int Immunopharmacol. 13:377. PubMed

IGEL-a2 cell line (IgE producing

IGEL-a2 cell line (IgE producing mouse hybridoma) stained with RME-1 PE



Description:

The RME-1 monoclonal antibody reacts with immunoglobulin E (IgE) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RME-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Other Names: Immunoglobulin E