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Safety Data Sheet (SDS) Purified Anti-mouse H-2D B Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse H-2D b Antibody
1157510 500 µg $220.00       
Clone: KH95
Isotype: Mouse (BALB/c) IgG2b, κ
Reactivity: Mouse b haplotype, Does not cross-react with other haplotypes (e.g., a, d, f, k, n, p, q, r, s, u, v)
Immunogen: C57BL/10 mouse skin graft and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, and Western blotting.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
2. Shao H, et al. 2005. J. Immunol. 175:1851.
3. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
4. Robb RJ, et al. 2012 Blood. 119:5898. PubMed
5. Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
6. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
7. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
8. Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed

C57BL/6 splenocytes stained with KH95

C57BL/6 splenocytes stained with KH95 PE

BALB/c splenocytes stained with KH95

BALB/c splenocytes stained with KH95 PE



Description:

The KH95 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Db haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.