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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse CD107b Mac-3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse CD107b (Mac-3) Antibody
1142550 100 µg $190.00       
Clone: M3/84
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
9. Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
10. Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
11. Nessler S, et al. 2007. Brain 130:2186. (IHC)

Thioglycolate-elicited BALB/c mouse peritoneal macrophages

Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 488


Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Other Names: CD107b, LAMP-2
Structure: 110 kD
Distribution: Macrophages, some myeloid cell lines
Function: Differentiation and activation
Antigen References:

1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.