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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD107b Mac-3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD107b (Mac-3) Antibody
1142555 25 µg $90.00       
1142560 100 µg $190.00       
Clone: M3/84
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IF - Validated
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per 106 cells in 100 µl volume. For immunofluorescence microscopy, a concentration range of 5-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
9. Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
10. Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
11. Nessler S, et al. 2007. Brain 130:2186. (IHC)
11. Tay SS, et al. 2014. PNAS. 111:2540. PubMed

Thioglycollate-elicited BALB/c mouse peritoneal macrophages

Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 647

Thioglycolate-elicited C57BL/6 mouse peritoneal cells

Thioglycolate-elicited C57BL/6 mouse peritoneal cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of anti-mouse CD107b (Mac-3) (clone M3/84) Alexa Fluor® 647 (blue) in blocking buffer for 3 hours at room temperature. Nuclei were counterstained with DAPI (red). The image was captured with 60X objective.



Description:

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Other Names: CD107b, LAMP-2
Structure: 110 kD
Distribution: Macrophages, some myeloid cell lines
Function: Differentiation and activation
Antigen References:

1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.


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