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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse TER-119/Erythroid Cells Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse TER-119/Erythroid Cells Antibody
1181090 100 µg $190.00       
Clone: TER-119
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Day-14 fetal liver cells from a C57BL/6 mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC , IF - Reported in the literature

Application Notes:

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays .

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
2. Vannucchi AM, et al. 2000. Blood 95:2559.
3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
4. Grisendi S, et al. 2005. Nature 437:147. (FC)
5. Bourdeau A, et al. 2007. Blood 109:4220.
6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
7. Heuser M, et al. 2007. Blood 110:1639. (FC)

C57BL/6 bone marrow cells stained

C57BL/6 bone marrow cells stained with TER-119 Alexa Fluor® 647 and anti-mouse CD45 FITC



Description:

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Other Names: Ly-76
Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Antigen References:

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.


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