Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PE Anti-mouse H-2Dk Antibody     Product Data Sheet (PDF)    
PE Anti-mouse H-2Dk Antibody
1151535 50 µg $100.00       
Clone: 15-5-5
Isotype: Mouse (C3H.SW) IgG2a, κ
Reactivity: Mouse k haplotype, Cross-reacts with H-2Kd and H-2f haplotypes. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s).
Immunogen: C3H mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Ozato K, et al. 1980. J. Immunol. 124:533.
2. Yamamoto M, et al. 1998. J. Immunol. 160:2188.
3. Xie X, et al. 2007. J. Virol.229:36.PubMed
4. Fang R, et al. 2007. Infect. Immun.75:3112.PubMed
3. Houlden BA, et al. 1991. J. Immunol. 146:425. (IP) PubMed

C3H/He mouse splenocytes were stained

C3H/He mouse splenocytes were stained with H-2Dk (clone 15-5-5) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).


The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Dk haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.