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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse Ly-51 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse Ly-51 Antibody
1141555 25 µg $90.00       
1141560 100 µg $190.00       
Clone: 6C3
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C57BL/6 mouse Pre-B lymphoma cell line (L1-2) plus Abelson murine leukemia virus-specific cytotoxic T-cell clones
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2 and immunohistochemical staining3-5, 7-8 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
2. Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
3. Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
4. Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
5. Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
6. Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
7. Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
8. Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 6C3 Alexa Fluor® 647


Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.

Other Names: 6C3/BP-1
Structure: Aminopeptidase A, 140 kD
Distribution: B progenitors, bone marrow stromal lines, thymic dendritic cells, cortical epithelial cells
Function: Aminopeptidase, may function in IL-7-driven proliferation of B cell precursors
Antigen References:

1. Goverman J, et al. 1997. Immunol. Today 18:204.
2. Morse HC, et al. 1987. J. Exp. Med. 165:920.
3. Sherwood P, et al. 1990. Int. Immunol. 2:399.
4. Lin Q, et al. 1998. J. Immunol. 160:4681.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.