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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD38 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD38 Antibody
1113580 100 µg $190.00       
Clone: 90
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse bone marrow pre-B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IF - Validated
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections, and induction of B cell proliferation1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescence microscopy, a concentration range of 2.5-10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Oliver AM, et al. 1997. J. Immunol. 158:1108.
2. Howard M, et al. 1993. Science 262:1056.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 90 Alexa Fluor® 647

C57BL/6 mouse frozen spleen section

C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS+ rat/mouse/hamster serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Alexa Fluor® 647 anti-mouse CD38 (red) (Clone 90), 5 µg/ml Alexa Fluor® 488 anti-mouse CD3ε (green) (clone 145-2C11), and 5 µg/ml of Alexa Fluor® 594 anti-mouse F4/80 Antibody (blue) (Clone BM8) overnight at 4°C. The image was captured by 10X objective.



Description:

CD38 is a 42 kD glycoprotein, also known as T10. It is an ADP-ribosyl hydrolase, expressed on B cells, NK cells, a subset of T cells, brain, muscle, and kidney. In mouse, CD38 expression is downregulated on germinal center B cells and plasma cells, whereas this is not the case for humans. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, as well as adhesion and metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Other Names: T10
Structure: ADP-ribosyl hydrolase, 42 kD
Distribution: B cells, NK cells, T cell subset, brain, muscle, kidney
Function: Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand Receptor: CD31, hyaluronic acid
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Shubinsky G, et al. 1997. Immunity 7:315.
3. Cesano A, et al. 1998. J. Immunol. 160:1106.
4. Cockayne DA, et al. 1998. Blood 92:1324.


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