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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD49e Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD49e Antibody
1119055 25 µg $90.00       
1119060 100 µg $190.00       
Clone: 5H10-27(MFR5)
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: (C57BL/6 x A/J)F1 mouse mast cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-β1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
7. Cuklerman E, et al. 2001. Science 294:1708. (IHC)
8. Jia W, et al. 2005. Blood 106:3854. (FC)

Balb/c mouse bone marrow stained

Balb/c mouse bone marrow stained with 5H10-27 Alexa Fluor® 647



Description:

CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Other Names: α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Structure: Integrin family, associates with integrin β1, 135 kD
Distribution: Thymocytes, activated T cells, mast cells
Function: Adhesion, costimulation
Ligand Receptor: Fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.


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