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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD49e Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD49e Antibody
1119055 25 µg $90.00       
1119060 100 µg $190.00       
Clone: 5H10-27(MFR5)
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: (C57BL/6 x A/J)F1 mouse mast cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-β1 costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
7. Cuklerman E, et al. 2001. Science 294:1708. (IHC)
8. Jia W, et al. 2005. Blood 106:3854. (FC)

Balb/c mouse bone marrow stained

Balb/c mouse bone marrow stained with 5H10-27 Alexa Fluor® 647


CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Other Names: α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Structure: Integrin family, associates with integrin β1, 135 kD
Distribution: Thymocytes, activated T cells, mast cells
Function: Adhesion, costimulation
Ligand Receptor: Fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.