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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-mouse/rat CD61 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-mouse/rat CD61 Antibody
1121555 25 µg $90.00       
Clone: 2C9.G2 (HMß3-1)
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat
Immunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-4, activation of αv β3 integrin signaling5, and immunohistochemical staining of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
2. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
3. Ashkar S, et al. 2000. Science 287:860. (Block)
4. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
5. Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
6. Carlson TR, et al. 2008.135:2193. PubMed
7. Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
8. Oliver CH, et al. 2012. Genes Dev. 26:1086. PubMed.
9. Jeanes AI, et al. 2012. J Biol Chem. 287:24103. PubMed.

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with 2C9.G2 Alexa Fluor® 488



Description:

CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv3 integrin or vitronectin receptor to CD31 (PECAM-1).

Other Names: β3 integrin, Integrin β3 chain, gpIIIa
Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand Receptor: Fibrinogen, von Willebrand factor, fibronectin
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell 65:359.
3. Felding-Habermann B, et al. 1993. Curr. Opinion Cell Biol. 5:864.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.