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Safety Data Sheet (SDS) Alexa Fluor 700 Anti-mouse CD451 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 700 Anti-mouse CD45.1 Antibody
1153615 25 µg $90.00       
1153620 100 µg $190.00       
Clone: A20
Isotype: Mouse (A.SW) IgG2a, κ
Reactivity: Mouse
Immunogen: SJL mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)), and immunofluorescence microscopy9.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
4. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC)
6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC)
7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC)
8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
9. Mende I, et al. 2006. Blood 107:1383. (IF, IHC, FC)
10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
20. Huang W, et al. 2014. J Leukoc Biol. 96:55. PubMed
21. Huang W, et al. 2014. J Immunol. 193:2267. PubMed
22. Lucca LE, et al. 2014. J Immunol. 193:3267. PubMed

SJL mouse splenocytes stained with

SJL mouse splenocytes stained with A20 Alexa Fluor® 700



Description:

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Other Names: T200, Ly-5.1, LCA
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
Function: Phosphatase, T and B cell activation
Ligand Receptor: Galectin-1, CD2, CD3, CD4
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.