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Safety Data Sheet (SDS) Purified Anti-mouse H-2Dk Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse H-2Dk Antibody
1151510 500 µg $210.00       
Clone: 15-5-5
Isotype: Mouse (C3H.SW) IgG2a, κ
Reactivity: Mouse k haplotype, Cross-reacts with H-2Kd and H-2f haplotypes. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s).
Immunogen: C3H mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling:

The antibody solution should be stored undiluted between 2°C and 8°C.

Application:

FC - Quality tested
IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ozato K, et al. 1980. J. Immunol. 124:533.
2. Yamamoto M, et al. 1998. J. Immunol. 160:2188.
3. Houlden BA, et al. 1991. J. Immunol. 146:425. (IP) PubMed

C3H/He mouse splenocytes were stained

C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a FITC.



Description:

The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Dk haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.