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Safety Data Sheet (SDS) FITC Anti-mouse CD24 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD24 Antibody
1109025 50 µg $65.00       
1109030 500 µg $245.00       
Clone: M1/69
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with M1/69 FITC



Description:

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Other Names: Heat Stable Antigen (HSA), Ly-52, Nectadrin
Structure: GPI-linked sialoglycoprotein, 35-45 kD
Distribution: B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
Function: Regulates B cell proliferation and differentiation
Ligand Receptor: P-selectin, CD24
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.