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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-Phosphotyrosine Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-Phosphotyrosine Antibody
2146540 100 µg $220.00       
Clone: PY20
Isotype: Mouse IgG2b, κ
Reactivity: All Species
Immunogen: KLH-conjugated phosphotyrosine
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunofluorescence microscopy3.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. NOTE: Do not use dilution buffer containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
6. Ramello MC, et al. 2014. Cell Death Dis. 6:5107. PubMed

Hydrogen peroxide stimulated EL4 cells

Hydrogen peroxide stimulated EL4 cells intracellularly stained with PY20 Alexa Fluor® 647



Description:

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Other Names:
Distribution: Phospho-Specific
Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.