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Safety Data Sheet (SDS) PE Anti-mouse/human CD11b Antibody     Product Data Sheet (PDF)    
PE Anti-mouse/human CD11b Antibody
1106035 50 µg $40.00       
1106040 200 µg $140.00       
Clone: M1/70
Isotype: Rat IgG2b, κ
Reactivity: Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Immunogen: C57BL/10 splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The CD11b antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC - Validated

Application Notes:

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
23. Kim DD, et al. 2008. Blood 112:1109. PubMed
24. Guo Y, et al. 2008. Blood 112:480. PubMed
25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
29. Hirako N, et al. 2014. PLoS One. 9:103282. PubMed
30. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
31. Textor A, et al. 2014. Cancer Res. 74:6796. PubMed
32. Jaworska J, et al. 2015. J Immunol. 194:325. PubMed
33. Xiao Z, et al. 2015. Cancer Lett. 360:302. PubMed
34. Li X, et al. 2015. PNAS. 112:6080. PubMed
35. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram) (gated on total cells).

Fixed whole mount mouse spleen

Fixed whole mount mouse spleen was stained with Alexa Fluor® 488 F4/80 (red) (clone BM8), Brilliant Violet 421™ CD3ε (green) (clone 145-2C11), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.



Description:

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Other Names: αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Structure: Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
Function: Adhesion, chemotaxis
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.