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Safety Data Sheet (SDS) Biotin Anti-rat CD43 Antibody     Product Data Sheet (PDF)    
Biotin Anti-rat CD43 Antibody
1614015 100 µg $125.00       
Clone: W3/13
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections1 and paraffin-embedded sections4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Barclay AN. 1981. Immunology 42:593.
2. Brown WRA, et al. 1981. Nature 289:456.
3. Forbes RC, et al. 1983. Transplantation 36:681.
4. Jung S, et al. 1994. Immunology 83:545.

LOU rat splenocytes stained with

LOU rat splenocytes stained with W3/13 biotin, followed by Sav-PE



Description:

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and W3/13, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, W3/13
Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130kD
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand Receptor: CD54, Siglec-1
Antigen References:

1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
2. Killeen A, et al. 1987. EMBO J. 6(13):4029.
3. Thurman EC, em>et al. 1998. Intl. Immunol. 10:691.
4. Onami TM, et al. 2002. J. Immunol. 168:6022.
5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
6. Jones AT, et al. 1994. J. Immunol. 153:3426.