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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD45RB Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD45RB Antibody
1116555 25 µg $90.00       
1116560 100 µg $190.00       
Clone: C363-16A
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Cloned mouse Th2 cell lines
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugatedAlexa Fluor® 647.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.

C57Bl/6 mouse splenocytes stained with

C57Bl/6 mouse splenocytes stained with C363-16A Alexa Fluor® 647



Description:

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Other Names:
Structure: Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells
Function: Phosphatase, T cell development and activation
Ligand Receptor: Galectin-1, CD2, CD3, CD4, Thy1
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.


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