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Safety Data Sheet (SDS) FITC Anti-mouse CD83 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD83 Antibody
1207525 25 µg $95.00       
1207530 100 µg $190.00       
Clone: Michel-19
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Recombinant mouse CD83 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone - fixed frozen sections4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
2. Fujimoto Y, et al. 2002. Cell 108:755.
3. Mott KR, et al. 2009. Virol J. 6:56. (FC) PubMed
4. Roland Cl, et al. 2009. Mol Cancer Res. 8:1761. (IHC) PubMed
5. Masuda Y, et al. 2010. Cancer Immunol Immunother. [Epub ahead of print] (FC) PubMed
6. Tze LE, et al. 2011. J Exp Med. PubMed
7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
8. Wallner S, et al. 2013. PLoS One. 8:65178. PubMed

mCD83 transfected cells stained with

mCD83 transfected cells stained with Michel-19 FITC



Description:

CD83 is a 45 kD type I transmembrane protein. It belongs to immunoglobulin superfamily and is expressed on mature dendritic cells and activated lymphocytes. CD83 is involved in the regulation of T cell development and immune response. Soluble form CD83 has been reported to inhibit dendritic cell maturation and dendritic cell-mediated T cell proliferation. Murine CD83 ligand has been found on B cells.

Other Names: HB15
Structure: Type I transmembrane protein, Ig superfamily, 45kD
Distribution: Mature dendritic cells, activated lymphocytes
Function: Regulate T cell development and immune response, soluble form CD83 inhibit dendritic cell maturation
Ligand Receptor: CD83 ligand
Antigen References:

1. Lechmann M, et al. 2005. Biochem. Biophys. Res. Commun. 329:132.
2. Kotxor N, et al. 2004. Immunobiology 209:129.
3. Leon F, et al. 2004. J. Immunol. 173:2995.
4. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
5. Fujimoto Y, et al. 2002. Cell 108:755.