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Safety Data Sheet (SDS) FITC Anti-mouse CD18 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD18 Antibody
1107025 50 µg $75.00       
Clone: M18/2
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse CTL glycoprotein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion2-6, and immunohistochemical staining7 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Sanchez-Madrid, et al. 1983. J. Exp. Med. 158:586. (IP, WB)
2. Zahalka MA, et al. 1993. J. Immunol. 150:4466. (IP, Block, Stim)
3. Zahalka MA, et al. 1994. Int. Immunol. 6:917. (Block, Stim)
4. Ishida Y, et al. 1994. Cell. Immunol. 155:414. (Block, Stim)
5. Isobe M, et al. 1992. Science 255:1125. (Block, Stim)
6. Driessens MHE, et al. 1996. J. Leukoc. Biol. 60:758. (Block, Stim)
7. Barlow SC, et al. 2004. Amer. J. Pathol. 165:1849. (IHC)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with M18/2 FITC



Description:

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.

Other Names: integrin β2, β2 integrin, ITGB2
Structure: Integrin family, associates with CD11a, CD11b, CD11c, 95 kD
Distribution: All leukocytes
Function: Adhesion
Ligand Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Wilson RW, et al. 1993. J. Immunol. 151:1571.