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Safety Data Sheet (SDS) Biotin Anti-mouse CD107a LAMP-1 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD107a (LAMP-1) Antibody
1208015 50 µg $80.00       
1208020 200 µg $170.00       
Clone: 1D4B
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: This monoclonal antibody was raised against NIH/3T3 mouse embryo fobroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

* Expected MW: 120 kD
WB Positive Control: Mouse 3T3 cell lysate and Mouse Spleen
IHC Positive Control: Mouse Pancreas, Colon, Kidney

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Thioglycollate-elicited BALB/c mouse peritoneal macrophages

Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with biotinylated 1D4B, followed by Sav-PE



Description:

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Other Names: Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1