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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD11b/c Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD11b/c Antibody
1609070 100 µg $190.00       
Clone: OX-42
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: Rat peritoneal macrophages.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Application References:

1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
3. Robinson AP, et al. 1986. Immunology 57:239. (Block)
4. Issekutz SE, et al. 1992. Immunology 76:655. (Block)
5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
6. Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
7. Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
8. Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
9. Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-42 Alexa Fluor® 647


The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, NK cells, and a subset of lymphocytes. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.

Other Names: Rat C3bi receptor, Rat CR3 complement receptor, ITGAM, ITGAX
Structure: Integrin family member
Distribution: Monocytes, granulocytes, macrophages, dendritic cells
Function: Receptor for C3bi
Ligand Receptor: Complement C3bi
Antigen References:

1. Robinson AP, et al. 1986. Immunology 57:239.
2. Barcaly AN. 1981 Immunology 42:593.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.