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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD45 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD45 Antibody
1611055 25 µg $90.00       
1611060 100 µg $190.00       
Clone: OX-1
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Enriched glycoprotein fraction from Wistar rat thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6 fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Application References:

1. Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
2. Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
3. Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
4. Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
5. Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
6. Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
7. Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
8. Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-1 Alexa Fluor® 647



Description:

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Other Names: Leukocyte common antigen, LCA, T200, Ly-5
Structure: Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution: All hematopoietic cells except erythrocytes and platelets
Function: T cell receptor and B cell receptor signal transduction
Ligand Receptor: Galectin-1, CD2, CD3, CD4
Antigen References:

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.


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