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Safety Data Sheet (SDS) Alexa Fluor 488 Anti-rat CD45RA Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 488 Anti-rat CD45RA Antibody
1611550 100 µg $190.00       
Clone: OX-33
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Leukocyte common antigen purified from rat splenocytes.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Application References:

1. Smith T, et al. 2000. Nature Med. 6:62. (IHC)

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-33 Alexa Fluor® 488



Description:

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Other Names: Leukocyte common antigen, high molecular weight form
Structure: Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution: Primarily on B lymphocytes
Function: B cell receptor signal transduction
Ligand Receptor: Galectin-1, CD2, CD3, CD4
Antigen References:

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.


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