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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD279 PD-1 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD279 (PD-1) Antibody
1145545 25 µg $95.00       
1145550 100 µg $260.00       
Clone: RMP1-30
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse PD-1 transfected BHK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The CD279 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported application (for the relevant formats) include: Functional assay.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed

Con A-stimulated (day-3) Balb/c mouse

Con A-stimulated (day-3) Balb/c mouse splenocytes stained with RMP1-30 PE/Cy7



Description:

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Other Names: Programmed Death-1, CD279
Structure: Ig superfamily, 50-55 kD
Distribution: Subset of double negative thymocytes, activated T and B cells
Function: Lymphocyte clonal selection, peripheral tolerance
Ligand Receptor: PD-L1 (B7-H1), PD-L2
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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