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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD86 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD86 Antibody
1601570 100 µg $190.00       
Clone: 24F
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IF, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)
2. Bluestone JA. 1995. Immunity 2:555. (IP, Block)
3. Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
4. Donius LR, et al. 2014. Immunobiology. 219:440. PubMed

ConA-stimulated (3 days) LOU rat

ConA-stimulated (3 days) LOU rat splenocytes stained with 24F Alexa Fluor® 647



Description:

CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Other Names: B7-2, B70
Structure: Ig superfamily, 80 kD
Distribution: Antigen presenting cells, activated B cells and T cells
Function: T and B cell costimulation
Ligand Receptor: CD28, CD152 (CTLA-4)
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.


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