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Safety Data Sheet (SDS) Purified Anti-mouse CD107b Mac-3 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD107b (Mac-3) Antibody
1142505 50 µg $75.00       
1142510 500 µg $215.00       
Clone: M3/84
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
3. Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
4. Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
6. Kano M, et al. 1998. Transplantation 65:837. (IHC)
7. Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
8. Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
9. Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
10. Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
11. Nessler S, et al. 2007. Brain 130:2186. (IHC)

Thioglycolate-elicited peritoneal macrophages stained with

Thioglycolate-elicited peritoneal macrophages stained with M3/84 PE



Description:

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Other Names: CD107b, LAMP-2
Structure: 110 kD
Distribution: Macrophages, some myeloid cell lines
Function: Differentiation and activation
Antigen References:

1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.