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Safety Data Sheet (SDS) Purified Anti-mouse/human CD282 TLR2 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse/human CD282 (TLR2) Antibody
1209005 25 µg $90.00       
1209010 100 µg $215.00       
Clone: T2.5
Isotype: Mouse IgG1, κ
Reactivity: Mouse, Human
Immunogen: Recombinant extracellular domain of murine TLR2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
Block, IHC, IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections, and inhibition of TLR2-dependent cell activation in vitro and in vivo1. Inhibition of activation occurs by binding to an epitope in the TLR2 extracellular domain which interferes with signaling-receptor complex formation 1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Meng G, et al. 2004. J. Clin. Invest. 113:1473. (Block, IHC, IP)



Description:

Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been indentified. Toll-like receptor 2 (TLR2), known as CD282, is one of the important receptors. It is expressed on monocytes/macrophages, dendritic cells, granulocytes, endothelial and epithelial cells. By association with TLR1 or TLR6 and CD36, TLR2 is central to the innate immune response to diverse set of pathogens, including several components of Gram-positive bacteria such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi. The T2.5 antibody reacts with both mouse and human TLR2. It is able to inhibit cell activation through blocking the interaction of TLR2 with its ligands.

Other Names: CD282, TLR-2, TLR2
Structure: Type I transmembrane protein characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain, associated with TLR1 or TLR6 and CD36
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells
Function: Activates innate host defense against diverse set of pathogens
Ligand Receptor: Microbial lipoproteins, peptidoglycans, lipoteichoic acid
Antigen References:

1. Hoebe K, et al. 2004. Nat. Immunol. 5:971.
2. Takeuchi O, et al. 2002. J. Immunol. 169:10.
3. Gantner BN, et al. 2003. J. Exp. Med. 197:1107.
4. Hoebe K, et al. 2005. Nature 433:523.
5. Meng GX, et al. 2004. J. Clin. Invest. 113:1473