Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse/human CD282 TLR2 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse/human CD282 (TLR2) Antibody
1209045 25 µg $125.00       
1209050 100 µg $270.00       
Clone: T2.5
Isotype: Mouse IgG1, κ
Reactivity: Mouse, Human
Immunogen: Recombinant extracellular domain of murine TLR2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections, and inhibition of TLR2-dependent cell activation in vitro and in vivo1. Inhibition of activation occurs by binding to an epitope in the TLR2 extracellular domain which interferes with signaling-receptor complex formation 1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Application References:

1. Meng G, et al. 2004. J. Clin. Invest. 113:1473. (Block, IHC, IP)

Human peripheral blood monocytes stained

Human peripheral blood monocytes stained with T2.5 Alexa Fluor® 647

Thioglycolate-elicited BALB/c mouse peritoneal macrophages

Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with T2.5 Alexa Fluor® 647



Description:

Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been indentified. Toll-like receptor 2 (TLR2), known as CD282, is one of the important receptors. It is expressed on monocytes/macrophages, dendritic cells, granulocytes, endothelial and epithelial cells. By association with TLR1 or TLR6 and CD36, TLR2 is central to the innate immune response to diverse set of pathogens, including several components of Gram-positive bacteria such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi. The T2.5 antibody reacts with both mouse and human TLR2. It is able to inhibit cell activation through blocking the interaction of TLR2 with its ligands.

Other Names: CD282, TLR-2, TLR2
Structure: Type I transmembrane protein characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain, associated with TLR1 or TLR6 and CD36
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells
Function: Activates innate host defense against diverse set of pathogens
Ligand Receptor: Microbial lipoproteins, peptidoglycans, lipoteichoic acid
Antigen References:

1. Hoebe K, et al. 2004. Nat. Immunol. 5:971.
2. Takeuchi O, et al. 2002. J. Immunol. 169:10.
3. Gantner BN, et al. 2003. J. Exp. Med. 197:1107.
4. Hoebe K, et al. 2005. Nature 433:523.
5. Meng GX, et al. 2004. J. Clin. Invest. 113:1473


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.