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Safety Data Sheet (SDS) PE Anti-human CD109 Antibody     Product Data Sheet (PDF)    
PE Anti-human CD109 Antibody
2216525 25 tests $95.00       
Clone: W7C5
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Giesert C, et al. In:Hematopoietic Stem Cells 2002:Genetics and function.
2. Orlic D, et al. 2003. Ann. New York Acad. Sci. 996:227.
3. Vogel W, et al. 2002. Haematologica 88:126.

Human peripheral blood monocytes stained

Human peripheral blood monocytes stained with W7C5 PE



Description:

The W7C5 monoclonal antibody recognizes human CD109 also known as the Gov platelet alloantigen. CD109 is a member of the alpha2-macroglobulin/complement gene family that is a GPI-linked cell surface antigen with a predicted molecular weight approximately 162 kD. CD109 contains a thioester motif characteristic of alpha-2-macroglobulin that undergoes autolytic cleavage under denaturing conditions. CD109 expression has been reported on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells. It is also widely expressed in the brain, uterus, heart, lung, and trachea of adults. The function of CD109 is largely unknown although it has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura.

Other Names: Gov platelet alloantigen
Structure: Member of the alpha2-macroglobulin/complement gene family. GPI-linked cell surface antigen, predicted molecular weight approximately 162 kD.
Distribution: Expressed on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells.
Function: Unknown; has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura
Antigen References:

1. Lin M, et al. 2002. Blood 99:1683.
2. Schuh AC, et al. 2002. Blood 99:1692.
3. Solomon KR, et al. 2004. Gene 327:171.