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Safety Data Sheet (SDS) FITC Anti-mouse CD28 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD28 Antibody
1210035 50 µg $70.00       
1210040 500 µg $225.00       
Clone: E18
Isotype: Mouse IgG2b, κ
Reactivity: Mouse
Immunogen: A20 cells and recombinant mCD28Ig
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Dennehy KM, et al. 2006. J. Immunol. 176:5725.
2. Reynolds AE, et al. 2015. J Immunol. 194:231. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 (145-2C11) PE and E18 FITC



Description:

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.

Other Names: Tp44, T44
Structure: Ig superfamily, 44 kD
Distribution: Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function: Costimulates T and NK cells
Ligand Receptor: CD80 (B7-1), CD86 (B7-2)
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.