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Safety Data Sheet (SDS) APC Anti-mouse CD62L Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD62L Antibody
1122055 25 µg $65.00       
1122060 100 µg $170.00       
Clone: MEL-14
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C3H/eb mouse B lymphoma 38C-13
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
7. Olver S, et al. 2006. Cancer Res. 66:571.
8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Lee JW, et al. 2006. Nature Immunol. 8:181.
12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).



Description:

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Other Names: L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Structure: Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution: Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Function: Lymphocyte homing to HEV, rolling on activated endothelium
Ligand Receptor: CD34, GlyCAM-1, MAdCAM-1
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.