Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) Pacific Blue Anti-mouse CD11c Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse CD11c Antibody
1186605 25 µg $90.00       
1186610 100 µg $190.00       
Clone: N418
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse spleen dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration: 0.5 mg/ml
Storage & Handling: The CD11c antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Granucci F, et al. 1997. J. Immunol. 159:1794.
2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
4. Ma XT, et al. 2006. Cancer Research 66:1169.
5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
19. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
20. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
21. Zaslona Z, et al. 2014. J Immunol. 193:4245. PubMed
22. Keller S, et al. 2014. J Control Release. 191:24. PubMed
23. Onishi M, et al. 2015. J Immunol. 194:2673. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with Pacific Blue™ N418



Description:

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Other Names: αX integrin, integrin αX chain, CR4, p150, ITGAX
Structure: Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution: Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function: Cellular adhesion
Ligand Receptor: iC3b, fibrinogen
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.