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Safety Data Sheet (SDS) Biotin Anti-mouse Ly-6A/E Sca-1 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse Ly-6A/E (Sca-1) Antibody
1212520 500 µg $220.00       
Clone: E13-161.7
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: mouse pre-T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.php?page=pro_sub_cat&action=search&criteria=D7">D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

 

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated E13-161.7, followed by Sav-PE



Description:

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.

Other Names: Sca-1
Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Antigen References:

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.