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Safety Data Sheet (SDS) Biotin Anti-human/mouse Integrin Beta7 Antibody     Product Data Sheet (PDF)    
Biotin Anti-human/mouse Integrin β7 Antibody
2206045 50 µg $75.00       
Clone: FIB504
Isotype: Rat IgG2a, κ
Workshop Number: VI 6T-101, VI A024
Reactivity: Human, Mouse, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The FIB504 antibody has been reported to react with mouse and human β7 integrin and to block β7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
2. Berlin C, et al. 1993. Cell 74:185.
3. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block)
4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block)
5. Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed
6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated FIB504, followed by Sav-PE



Description:

Integrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,αIEL/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.

Other Names: β7 Integrin, integrin βp, ITGB7
Structure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution: CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
Function: Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand Receptor: CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
Antigen References:

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nautre 372:190.