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Safety Data Sheet (SDS) Biotin Anti-mouse CD229 Ly-9 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD229 (Ly-9) Antibody
1214515 50 µg $75.00       
Clone: Ly9ab3
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: AHK cells transiently transfected with mouse CD229 (Ly-9)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Romero X, et al. 2005. J. Immunol. 174:7033.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated Ly9ab3, followed by Sav-PE



Description:

CD229 is a 100-120 kD glycoprotein. It is a member of the SLAM family, a CD2 subset of the Ig superfamily, known as Ly9 or SLAMF3. CD229 is expressed on T cells, B cells, NK cells, and thymocytes. It functions as a homophilic adhesion molecule through binding to CD229 itself. The cytoplasmic tail of CD229 binds to SAP and Grb2 proteins. CD229 is involved in enhancing T cell activation and Th2 polarization.

Other Names: Ly9, SLAMF3
Structure: 100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily
Distribution: T cells, B cells, NK cells, thymocytes
Function: Homophilic adhesion, T cell activation, Th2 cell polarization
Ligand Receptor: CD229
Antigen References:

1. Sandrin MS, et al. 1996. Immunogenetics 43:13.
2. de la Fuente MA, et al. 2001. Blood 97:3513.
3. Romero X, et al. 2005. J. Immunol. 174:7033.
4. Martin M, et al. 2005. J. Immunol. 174:5977.
5. Graham DB, et al. 2006. J. Immunol. 176:291.