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Safety Data Sheet (SDS) Purified Anti-mouse I-Ak Abetak Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse I-Ak (Aβk) Antibody
1149510 500 µg $210.00       
Clone: 10-3.6
Isotype: Mouse (CWB) IgG2a, κ
Reactivity: Mouse k haplotype, Cross-reacts with H-2 f,r,s haplotypes. Does not cross react with other haplotypes (e.g. b, d, p, q).
Immunogen: C3H mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
2. Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
4. Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
5. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
6. Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
7. Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)

C3H mouse splenocytes stained with

C3H mouse splenocytes stained with 10-3.6 purified, followed by anti-mouse IgG2a FITC



Description:

The 10-3.6 antibody reacts with the β chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens and I-Ag7 of NOD mice; it does not react with other haplotypes (e.g., b, d, p, q).

Other Names: MHC class II
Structure: β chain of MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.