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Safety Data Sheet (SDS) FITC Anti-mouse CD80 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD80 Antibody
1123525 50 µg $65.00       
1123530 500 µg $180.00       
Clone: 16-10A1
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Cross-Reactivity: Dog (Canine)
Immunogen: CHO cell line transfected with mouse B7 (CD80)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
5. Ma XT, et al. 2006. Cancer Res. 66:1169.
6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
11. Liang Y, et al. 2014. J. Immunol. 192:1277. PubMed
12. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
13. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
14. Li XL, et al. 2014. PLoS One. 9:108192. PubMed

LPS-stimulated (3 days) BALB/c splenocytes

LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC



Description:

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Other Names: B7-1, B7, Ly-53
Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.