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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD3 Antibody
1607040 100 µg $190.00       
Clone: 1F4
Isotype: Mouse IgM, κ
Reactivity: Rat
Immunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was conjugated with Alexa Fluor® 647 under optimal conditions, and is at >85% purity.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
2. Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
3. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
4. Baba T, et al. 2006. Blood 107:2004. (IHC)
5. Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
6. Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with 1F4 Alexa Fluor® 647


CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. 

Other Names: T cell antigen receptor complex, T3
Structure: Ig superfamily, approximately 20-25 kD
Distribution: Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor: Peptide antigen/MHC complex
Antigen References:

1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.